Scientific Applications

Your research

The founders and scientists at abberior not only laid the groundwork for superresolution microscopy, they also were among the first to develop its application to biological, medical and material science. And they are always available to offer guidance should you need it. You can count on first-class advice when it comes to getting the most out of your microscope!

our solution

FAQ 28

“Which abberior microscope should I get?”

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Light microscopy allows to analyze dynamics and structures within living and unperturbed cells and tissues. This is one of the key aspects that differentiates it from other types of microscopy.

For live cell imaging sessions, a range of features as water immersion lenses, an autofocus unit and environmental control chambers are crucial, but the key to extended life cell imaging is minimizing the light dose which is applied to the sample.

abberior offers several unique hard- and software features which facilitates imaging of living cells and tissues.

FAQ 06

“What is required for a microscope for live cell imaging?”

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Cell biology comprises numerous disciplines such as organelle biology, cell division, cytoskeleton, genome biology, differentiation, cellular dynamics and transport.

Research in modern cell biology is frequently based on living and fixed cultured cells using a variety of research approaches and tools, where light microscopy is one of the most important ones for this discipline.

Because the size of organelles and other intracellular structures is often below the resolution limit of conventional light microscopy, superresolution techniques such as STED and MINFLUX are now frequently used for research in cell biology.

abberior offers several unique microscope features which facilitate imaging of fixed and living cell samples over extended time periods at highest possible resolution:

  • MINFLUX imaging mode for resolutions down to 2 nm
  • MINFLUX single particle tracking with up to 10 kHz, i.e. 1 data point every 100 µs
  • Adaptive illumination for superresolution STED imaging at minimal light dosages.
  • Lifetime imaging and advanced time-gating.
  • Pulsed high-power STED lasers for best resolutions with 2D and 3D superresolution nanoscopy
  • Detectors with superior detection efficiency (up to 65%)

Research in neuroscience is centered around the structure and function of neurons and the nervous system. It combines disciplines like (electro-)physiology, anatomy, molecular, and developmental biology, to name a few.

Often, neuroscience hinges on imaging of cultured neuronal cells and tissue sections, both fixed and alive. Light microscopy is a valuable tool here, since it allows the analysis of the structure, function and interaction of neuronal cells and systems in an intact and even living state. Because the size of vesicles, synapses, spines and other neuronal structures is frequently below the resolution limit of conventional light microscopy, superresolution methods such as STED and MINFLUX nanoscopy are frequently used for neuroscience research.

abberior offers several unique microscope features which facilitate imaging of fixed and living neurobiological samples over extended time periods at the highest possible resolution:

  • MINFLUX imaging mode for resolutions donw to 2 nm
  • MINFLUX single particle tracking with up to 10 kHz, i.e. 1 data point every 100 µs
  • Adaptive illumination for superresolution STED imaging at minimal light dosages.
  • Lifetime imaging and advanced time-gating.
  • Pulsed high-power STED lasers for best resolutions with 2D and 3D superresolution imaging
  • Detectors with superior detection efficiency (up to 65%)

Virology studies the function, genetics and structure of viruses. In addition to that, components of viroids, infection and maturation of virus particles within their host cells are important topics in virology. In the past, virus particles could only be imaged using electron microscopy, due to their small size. Today, superresolution approaches such as STED and MINFLUX nanoscopy facilitate virology research in living and fixed systems.

abberior offers several unique microscope features which facilitate imaging of virus particles at the highest possible resolution:

  • MINFLUX imaging mode for resolutions down to 2 nm
  • MINFLUX single particle tracking with up to 10 kHz, i.e. 1 data point every 100 µs
  • Adaptive illumination for superresolution STED imaging at minimal light dosages.
  • Lifetime imaging and advanced time-gating.
  • Pulsed high-power STED lasers for best resolutions with 2D and 3D superresolution imaging
  • Detectors with superior detection efficiency (up to 65%)

Microbiology comprises areas such as bacteriology and mycology. During the foundation of this field, microorganisms like bacteria and fungi were characterized using transmitted light microscopy, whereas today, the majority of studies uses fluorescence light microscopy. Microbiologists investigate  gene expression, host-pathogen interactions, the live cycle of organisms, infection biology, bacterial cell biology and many things more.

The size of many microorganisms is in the range of the resolution limit of conventional light microscopy. Thus, the analysis of substructures often is not possible without the resolving power of STED and MINFLUX nanoscopy.

abberior offers several unique microscope features which facilitate imaging of fixed and living neurobiological samples over extended time periods at highest possible resolution:

  • MINFLUX imaging mode for resolutions down to 2 nm
  • MINFLUX single particle tracking with up to 10 kHz, i.e. 1 data point every 100 µs
  • Adaptive illumination for superresolution STED imaging at minimal light dosages.
  • Lifetime imaging and advanced time-gating.
  • Pulsed high-power STED lasers for best resolutions in 2D and 3D superresolution nanoscopy
  • Detectors with superior detection efficiency (up to 65%)

 

Zoology ist the study of animals and comprises developmental biology, genetics, biochemisty and physiology. Various experimental methods are used for zoology research, most frequently light microscopy. In zoology, imaging typically spans several lenght scales: imaging frequently starts at the whole organism, continues with tissues or organs, then, individual cells are analyzed, and at the final level of resolution, intracellular features such as protein assemblies or organelles are observed. Obstacles in zoological imaging are often intransparent structures or autofluorescent background, which stems from intransparent cells or the cuticle.

abberior offers several unique microscope features which facilitate imaging of zoological samples at several levels of resolution:

  • MINFLUX imaging mode for resolutions down to 2 nm
  • MINFLUX single particle tracking with up to 10 kHz, i.e. 1 data point every 100 µs
  • Adaptive illumination for superresolution STED imaging at minimal light dosages.
  • Lifetime imaging and advanced time-gating.
  • Pulsed high-power STED lasers for best resolutions with 2D and 3D superresolution nanoscopy
  • Detectors with superior detection efficiency (up to 65%)

Modern plant sciences comprise breeding research, (plant-)developmental biology, genetics, biochemisty and (plant-)physiology. Various experimental methods are used in plant research and light microscopy is prominent among them.  In plant science, imaging typically spans several lenght scales: imaging frequently starts at the scale of a whole organism, continues with tissue or sections, then, individual cells are analyzed, and at the final level of resolution intracellular features such as protein assemblies or organelles are observed. Obstacles in plant imaging is often the large autofluorescent background, which stems from the cuticle or chloroplasts.

abberior offers several unique microscope features which facilitate imaging of plant samples at several levels of resolution:

  • MINFLUX Imaging mode for localization precisions down to 2 nm
  • MINFLUX Single particle tracking with up to 10 kHz, i.e. 1 data point every 100 µs
  • Adaptive Illumination for confocal and superresolution STED nanoscopy at minimal light dose.
  • Advance Time gating for confocal and superresolution STED nanoscopy at minimal light dose @ reduced light dose.
  • Advance Time gating for confocal and superresolution STED nanoscopy for reducing autofluorescent background.
  • Pulsed High power STED lasers for best resolution in 2D and 3D superresolution imaging
  • Detectors with superior detection efficiency (up to 65%)

Biophysics is a highly diverse field of research. The light microscopic investigation of biomaterials, proteins and nucleic acids are hot topics in biophysics, to name a few. For these experiments, very precise and accurate instruments are required.

abberior offers several microscope features which facilitate biophysics research at several levels of resolution:

  • MINFLUX imaging mode for resolutions down to 2 nm
  • MINFLUX single particle tracking with up to 10 kHz, i.e. 1 data point every 100 µs
  • Adaptive illumination for superresolution STED imaging at minimal light dosages.
  • Lifetime imaging and advanced time-gating.
  • Pulsed high-power STED lasers for best resolutions with 2D and 3D superresolution nanoscopy
  • Detectors with superior detection efficiency (up to 65%)
  • FLIM, PLIM
  • Customization of the instrument’s hard- and software

Physiology is centered around the analysis of structure and function of cells and tissues. It combines disciplines such as (electro-)physiology, anatomy and molecular biology.

Vastly different length scales have to be taken into account: from the whole organism, over tissues or organs, then individual cells, and – at the final level of resolution – intracellular features such as protein assemblies or organelles.

Light microscopy is one of the most important techniques for the analysis of the structure, function and interaction of cells and systems. Because the size of (sub-)cellular structures is often below the resolution limit of conventional light microscopy, superresolution approaches like  STED and MINFLUX nanoscopy are frequently used for physiology research.

abberior offers several unique microscope features which facilitate imaging of fixed and living samples over extended time periods at highest possible resolution:

  • Adaptive Optics for superresolution tissue imaging at extended depths.
  • MINFLUX imaging mode for resolutions down to 2 nm
  • MINFLUX single particle tracking with up to 10 kHz, i.e. 1 data point every 100 µs
  • Adaptive illumination for superresolution STED imaging at minimal light dosages.

Membrane biology is a field of research that addresses several questions around the composition, separation, dynamics and adaptation of membranes and membranous compartments. Besides artificial membrane systems in various compositions and layouts, cultured cells are model systems for membrane sciences. Typically, dynamic ‘living’ systems are investigated. Because of this, light dosage and light toxicity are important topics for the microscopic investigation of membranes and care has to be taken to lower both as much as possible.

For membrane biology, very precise and accurate instrument are frequently required.

abberior offers several microscope features which facilitate research in membrane biology at several levels of resolution:

  • MINFLUX imaging mode for resolutions down to 2 nm
  • MINFLUX single particle tracking with up to 10 kHz, i.e. 1 data point every 100 µs
  • Adaptive illumination for superresolution STED nanoscopy at minimal light dosages.
  • Lifetime imaging and advanced time-gating.
  • Pulsed high-power STED lasers for best resolutions with 2D and 3D superresolution imaging
  • Detectors with superior detection efficiency (up to 65%)
  • FLIM, PLIM
  • Customization of the instrument’s hard- and software

Analysis of structure and function of existing and development of new materials are central objectives in material science. Due to the small size of the material’s building blocks, conventional light microscopy is frequently not sufficient. For this reason, electron microscopy and near field microscopy were important tools for material science. Today, 2D and 3D superresolution approaches like STED and MINFLUX facilitate material science research in various experimental systems. Most importantly, due to the use of light microscopy, the analysis of the materials can be done without sectioning and without changing the properties of the materials.

abberior offers several unique microscope features which facilitate material sciences imaging at highest possible resolution:

  • MINFLUX imaging mode for resolutions down to 2 nm
  • MINFLUX single particle tracking with up to 10 kHz, i.e. 1 data point every 100 µs
  • Adaptive illumination for superresolution STED imaging at minimal light dosages.
  • Lifetime imaging and advanced time-gating.
  • Pulsed high-power STED lasers for best resolutions with 2D and 3D superresolution nanoscopy
  • Detectors with superior detection efficiency (up to 65%)

You don’t specialize in a particular application?

You need a versatile workhorse that can handle routine applications as well as special imaging problems?

Ease-of-use is as important to you as extraordinary performance?

Don’t hesitate to contact us, and our experienced specialists will tailor a microscope for you that exactly fits your needs.

abberior is renowned for the ability deliver microscope systems with an extraordinary degree of customization. Whatever microscopy application you have, whatever is required to image your sample, get in touch with us and we will start up the mightiest development team in the world of microscopy to give you exactly what you need. We rock the mic right!