STEDYCON
The STEDYCON upgrades your existing widefield system to a confocal microscope and STED nanoscope with a resolution down to 30 nm. All that’s required is a free camera port and a good objective lens. With its super-intuitive user interface, the STEDYCON provides an intelligent microscope platform that enables everyone to acquire superb superresolution images after only minutes of training. Define your experiment and enjoy fine imaging at the push of a button!
a class of its own
Description
Two proteins in the Golgi apparatus were immunolabelled using primary antibodies specific for GM130 and Giantin and secondary antibodies coupled to Abberior STAR 580 and Abberior STAR 635P. Shown is RAW DATA. Images were acquired using a STEDYCON attached to a Zeiss Axio Imager Z2.
Description
Confocal Imaging of a large sample region. Shown is a 2.8 mm x 2.5 mm region, acquired using 35 by 29 tiles using the STEADYFOCUS and stitched with SVI Huygens. Sample: mammalian cells immunolabelled for the mitochondrial protein TOM20 (STAR RED, red), double-stranded DNA to visualize mitochondrial DNA (STAR ORANGE, green), Phalloidin to label F-actin (STAR GREEN, gray), and DAPI to label nuclei. Sample preparation by Abberior GmbH.
Description
Immunolabelling of βIV-spectrin, a scaffolding protein of the axon initial segment, using a self-made primary antibody and Alexa594 secondary antibody. Cryo-section of mouse neocortex cut at 20 µm. Images were acquired using a STEDYCON attached to a Zeiss AxioImager Z2. The sample was kindly provided by Dr. Maren Engelhardt, Institute of Neuroanatomy, Medical Faculty Mannheim, Heidelberg University.
Description
Pollen autofluorescence imaged with a STEDYCON on an IX83 microscope frame. z-stacks on 95 tiles were acquired and stitched with SVI Huygens.
Description
Body wall preparation of a 3rd instar Drosophila melanogaster larva. Synapses at the neuromuscular junction are visualized by immunostaining against Bruchpilot (Brp), an integral component of active zones in Drosophila. Images were acquired on a STEDYCON attached to an Olympus IX81. Images were kindly provided by Dr. Nadine Ehmann, Institute of Physiology, Neurophysiology, University of Würzburg
Description
Maximum intensity projection of a z-stack showing nuclear pore complex protein (red, Abberior STAR RED) and peroxisomes (cyan, Alexa 594) in mammalian cells. Imaged with Abberior Instruments’ STEDYCON and deconvolved with SVI Huygens optimized for STEDYCON.
Description
Confocal acquisition of Convallaria rhizome (cross-section) with a 20x oil objective; 3 channels, maximum intensity projection. The image consists of 14 z-planes in 9 by 9 tile pattern comprising a total area of 3.2 mm x 3.2 mm, stitched using SVI Huygens. Shown is a maximum intensity projection.
Description
Centrosome linker. U2OS cells in which the centrosome-linker-protein rootletin was immunolabelled using secondary antibodies coupled to Abberior STAR RED. The sample was prepared by R. Vlijm at MPI for Medical Research, Heidelberg, Germany. Imaged with Abberior Instruments’ STEDYCON and deconvolved with SVI Huygens optimized for STEDYCON.
Description
Drosophila ovariole stained with Abberior LIVE 560 DNA, showing the different cell types of the egg chamber. The image was stitched using SVI Huygens.
Description
Primary hippocampal neurons with cytoskeleton proteins labeled (magenta, alpha-Adducin, Abberior STAR 635P and green, ßII spectrin, Alexa 594). Imaged with Abberior Instruments’ STEDYCON and deconvolved with SVI Huygens optimized for STEDYCON.
Description
Two proteins in the Golgi apparatus were immunolabelled using primary antibodies specific for GM130 and Giantin and secondary antibodies coupled to Abberior STAR 580 and Abberior STAR 635P. Shown is RAW DATA. Images were acquired using a STEDYCON attached to a Zeiss Axio Imager Z2.
Description
Confocal Imaging of a large sample region. Shown is a 2.8 mm x 2.5 mm region, acquired using 35 by 29 tiles using the STEADYFOCUS and stitched with SVI Huygens. Sample: mammalian cells immunolabelled for the mitochondrial protein TOM20 (STAR RED, red), double-stranded DNA to visualize mitochondrial DNA (STAR ORANGE, green), Phalloidin to label F-actin (STAR GREEN, gray), and DAPI to label nuclei. Sample preparation by Abberior GmbH.
Description
Immunolabelling of βIV-spectrin, a scaffolding protein of the axon initial segment, using a self-made primary antibody and Alexa594 secondary antibody. Cryo-section of mouse neocortex cut at 20 µm. Images were acquired using a STEDYCON attached to a Zeiss AxioImager Z2. The sample was kindly provided by Dr. Maren Engelhardt, Institute of Neuroanatomy, Medical Faculty Mannheim, Heidelberg University.
Description
Pollen autofluorescence imaged with a STEDYCON on an IX83 microscope frame. z-stacks on 95 tiles were acquired and stitched with SVI Huygens.
Description
Body wall preparation of a 3rd instar Drosophila melanogaster larva. Synapses at the neuromuscular junction are visualized by immunostaining against Bruchpilot (Brp), an integral component of active zones in Drosophila. Images were acquired on a STEDYCON attached to an Olympus IX81. Images were kindly provided by Dr. Nadine Ehmann, Institute of Physiology, Neurophysiology, University of Würzburg
Description
Maximum intensity projection of a z-stack showing nuclear pore complex protein (red, Abberior STAR RED) and peroxisomes (cyan, Alexa 594) in mammalian cells. Imaged with Abberior Instruments’ STEDYCON and deconvolved with SVI Huygens optimized for STEDYCON.
Description
Confocal acquisition of Convallaria rhizome (cross-section) with a 20x oil objective; 3 channels, maximum intensity projection. The image consists of 14 z-planes in 9 by 9 tile pattern comprising a total area of 3.2 mm x 3.2 mm, stitched using SVI Huygens. Shown is a maximum intensity projection.
Description
Description
Centrosome linker. U2OS cells in which the centrosome-linker-protein rootletin was immunolabelled using secondary antibodies coupled to Abberior STAR RED. The sample was prepared by R. Vlijm at MPI for Medical Research, Heidelberg, Germany. Imaged with Abberior Instruments’ STEDYCON and deconvolved with SVI Huygens optimized for STEDYCON.
Description
Drosophila ovariole stained with Abberior LIVE 560 DNA, showing the different cell types of the egg chamber. The image was stitched using SVI Huygens.
Description
Primary hippocampal neurons with cytoskeleton proteins labeled (magenta, alpha-Adducin, Abberior STAR 635P and green, ßII spectrin, Alexa 594). Imaged with Abberior Instruments’ STEDYCON and deconvolved with SVI Huygens optimized for STEDYCON.
STEDYCON
super small and super stable
The incredibly compact STEDYCON has the size of a shoebox, yet it is a full-fledged confocal and STED microscope. It fits on every microscope frame with a camera port. Get in touch with us to upgrade your system!
The STEDYCON is service and maintenance-free: The laser beams are aligned by design. So, there is no need to align the system, ever, and it works reliably every day.
STEDYCON
extremely user-friendly
Save your time with the STEDYCON software: only minutes of training are required to acquire STED and confocal images. Thanks to the powerful dye database behind, only minimal user input is needed, and even with unkown samples, you get with 3 clicks to the first STED image!
Our software is browser-based, so run it from any device, from any platform, from anywhere!
STEADYFOCUS
in focus, forever
The STEADYFOCUS laser runs side-by side with the imaging beam path. No dichroics are required and there is absolutely no interference with imaging.
The autofocus laser is coupled into the beam path using a dichroic mirror, which interferes with imaging.
Our STEADYFOCUS keeps the focus position stable. Forever. During STED imaging, when browsing through the sample, or for hours of time-lapse imaging, there is no need for refocussing, the STEADYFOCUS does the work for you. And everything you need for it is integrated inside the STEDYCON head, so it works on upright microscopes, too!
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STEADYFOCUS
tiling and stitching is a piece of cake
Discover your samples! Acquire a quick tiled overview as a basis for tiled scans with overlap or for multi-position imaging. Define you experiment, let the microscope image for you, with active drift correction thanks to our STEADYFOCUS!
After tiled image acquisition, send over the data to Huygens, for easy, fast and reliable stitching – all needed parameters are automatically transferred.
Have a closer look here! In this joint online seminar abberior and SVI present tiling and stitching with STEDYCON and Huygens:
Get the video.
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Multipositioning
let our STEDYCON do the work
Discover your whole sample, and then take your time to define your experiment – at which position would you like to acquire a z-stack, or a single image, or a tiled scan? Then let the microscope do the imaging for you. Increase your working efficiency, let it run over night, or already prepare your next experiment! It saves time and endless clicks …
