FLIM – fluorescence lifetime imaging
At abberior, we not only work on increasing the amount of spatial resolution information you get from your samples. We’ve integrated lifetime imaging into our systems, to give you the best data not only in space, but also in time. Because at abberior, every dimension counts.
adding the 5th dimension
Many eyes see more than one. The MATRIX detector drastically improves signal-to-background ratio, resolution, and dynamic range.
Brings down the light dose on your sample and lables dramatically. Key ingredient for volume and live-cell superresolution.
FLIM image of tubulin labeled with Atto647N and vimentin labeled with abberior STAR 635P. The two labels (Atto647N and abberior STAR 635P) have the same excitation spectra, but different lifetimes. Tubulin (red, short lifetime) and vimentin (green, long lifetime) can successfully be separated in the STED FLIM recording via their lifetimes.
it’s high time to add it
All abberior microscopes, including the STEDYCON, can be equipped with fully integrated time-correlated single-photon counting (TCSPC) electronics for lifetime imaging, based on Becker & Hickl or PicoQuant hardware.
This way, lifetime data can be recorded simultaneously with standard intensity measurements, and can be written to disk for later analysis, or displayed as lifetime histograms or color-coded lifetime value. As is typical for abberior, raw data is always kept. For example, you can export an image where local lifetime is displayed in pseudo-colors, and you can inspect and save a lifetime stack, which e.g. for a 2D image carries the complete lifetime histogram in a third dimension.
precise timing of life
Lifetime imaging with our pulsed lasers and TCSPC electronics gives you the possibility to sense the chemical environment and adds an extra dimension to your image data. You can use FLIM to gain local information about pH or the concentration of dyes for another type of contrast next to labeling with different dyes.
clear-cut separation of dyes with spectral overlap
FLIM allows to separate fluorescence dyes by their lifetime, even when they fluoresce at the same wavelength. This means that STED and confocal imaging get additional channels!
- Confocal and STED FLIM imaging
- Online display of STED FLIM images during the measurement
- Online lifetime calculation and/or dye separation based on lifetime data
- Full software integration with live evaluation of data
Fluorescence lifetime imaging adds an extra layer of information
“Why do we usually recommend APDs in our microscopes and why aren’t we worried about the supposedly lower dynamic range?”
Dr. Andreas Schönle
3D-STED and aberration control, the easy way
Even better resolution through dynamic adjustment of the STED intensity