FLIM

FLIM – fluorescence lifetime imaging

At abberior, we not only work on increasing the amount of spatial resolution information you get from your samples. We’ve integrated lifetime imaging into our systems, to give you the best data not only in space, but also in time. Because at abberior, every dimension counts. 

adding the 5th dimension

MATRIX Detector

Many eyes see more than one. The MATRIX detector drastically improves signal-to-background ratio, resolution, and dynamic range.

Details >

Adaptive Illumination

Brings down the light dose on your sample and lables dramatically. Key ingredient for volume and live-cell superresolution.

Details >

Adaptive Optics

Puts every beam in perfect shape

Details >

EASY3D

3D-STED and aberration control, the easy way

Details >

RAINBOW Detection

Continuously variable spectral detection.

Details >

STED Lasers

All-pulsed STED lasers for maximum resolution and minimal bleaching.

Details >

Autoalignment

Every beam in order, including pinhole and STED-shape

Details >

Autofocus

Continuous focus-lock, even with STED

Details >

Excitation Lasers

Pulsed excitation lasers for virtually every wavelength.

Details >

FLIM

Lifetime imaging at its best

Details >

Accessories

Things to make your life easier

Details >

Custom Solutions

We offer solutions for even the most challenging applications. Everything that can be done, we will do.

Details >

FLIM

it’s high time to add it

All abberior microscopes, including the STEDYCON, can be equipped with fully integrated time-correlated single-photon counting (TCSPC) electronics for lifetime imaging, based on Becker & Hickl or PicoQuant hardware.

This way, lifetime data can be recorded simultaneously with standard intensity measurements, and can be written to disk for later analysis, or displayed as lifetime histograms or color-coded lifetime value. As is typical for abberior, raw data is always kept. For example, you can export an image where local lifetime is displayed in pseudo-colors, and you can inspect and save a lifetime stack, which e.g. for a 2D image carries the complete lifetime histogram in a third dimension.

FLIM

precise timing of life

Lifetime imaging with our pulsed lasers and TCSPC electronics gives you the possibility to sense the chemical environment and adds an extra dimension to your image data. You can use FLIM to gain local information about pH or the concentration of dyes for another type of contrast next to labeling with different dyes.

FLIM

clear-cut separation of dyes with spectral overlap

FLIM allows to separate fluorescence dyes by their lifetime, even when they fluoresce at the same wavelength. This means that STED and confocal imaging get additional channels!

  • Confocal and STED FLIM imaging
  • Online display of STED FLIM images during the measurement
  • Online lifetime calculation and/or dye separation based on lifetime data
  • Full software integration with live evaluation of data

Fluorescence lifetime imaging adds an extra layer of information

“Why do we usually recommend APDs in our microscopes and why aren’t we worried about the supposedly lower dynamic range?”

Dr. Andreas Schönle

Details >

EASY3D

3D-STED and aberration control, the easy way

Details >

DYMIN

Even better resolution through dynamic adjustment of the STED intensity

Details >